ssr_primer
Primer Details for TRINITY_DN81746_c2_g1_i5 |
|---|
| (T)10attaa(T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTGCATGACAAGTCTCA | AAGCTCCCTACAGCACACAC | Start: 1998 |
| Tm | 59.966 | 59.965 | End: 2202 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | ACGCTGCATGACAAGTCTCA | GCAGTTACTTCCTCAGCCGT | Start: 1998 |
| Tm | 59.966 | 60.037 | End: 2223 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | ACGCTGCATGACAAGTCTCA | CATGTGCCTTCCCCAACTCT | Start: 1998 |
| Tm | 59.966 | 59.961 | End: 2114 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | AAGTCTGCAGGAATGGTGGG | TGAGACTTGTCATGCAGCGT | Start: 1913 |
| Tm | 59.961 | 59.966 | End: 2017 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | AGAGTTGGGGAAGGCACATG | AAGCTCCCTACAGCACACAC | Start: 2095 |
| Tm | 59.961 | 59.965 | End: 2202 |
| Size | 20 | 20 | 108 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTGCATGACAAGTCTCA | AAGCTCCCTACAGCACACAC | Start: 1998 |
| Tm | 59.966 | 59.965 | End: 2202 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | ACGCTGCATGACAAGTCTCA | GCAGTTACTTCCTCAGCCGT | Start: 1998 |
| Tm | 59.966 | 60.037 | End: 2223 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | ACGCTGCATGACAAGTCTCA | CATGTGCCTTCCCCAACTCT | Start: 1998 |
| Tm | 59.966 | 59.961 | End: 2114 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | AAGTCTGCAGGAATGGTGGG | TGAGACTTGTCATGCAGCGT | Start: 1913 |
| Tm | 59.961 | 59.966 | End: 2017 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | AGAGTTGGGGAAGGCACATG | AAGCTCCCTACAGCACACAC | Start: 2095 |
| Tm | 59.961 | 59.965 | End: 2202 |
| Size | 20 | 20 | 108 |
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