Primer Details for TRINITY_DN81716_c4_g1_i3 |
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| (A)12taaat(A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCTGAAATTCCGAGCCCA | TGGAAGTAAACTAAACCTAGGTACGT | Start: 52 |
| Tm | 60.035 | 59.507 | End: 171 |
| Size | 20 | 26 | 120 |
| Primer 2 details |
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| Sequence | GAAAAGCCGTCAATGCGACA | TGGAAGTAAACTAAACCTAGGTACGT | Start: 29 |
| Tm | 59.763 | 59.507 | End: 171 |
| Size | 20 | 26 | 143 |
| Primer 3 details |
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| Sequence | AAAGCCGTCAATGCGACAAG | TGGAAGTAAACTAAACCTAGGTACGT | Start: 31 |
| Tm | 59.762 | 59.507 | End: 171 |
| Size | 20 | 26 | 141 |
| Primer 4 details |
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| Sequence | AAGGAAAAGCCGTCAATGCG | TGGAAGTAAACTAAACCTAGGTACGT | Start: 26 |
| Tm | 59.759 | 59.507 | End: 171 |
| Size | 20 | 26 | 146 |
| Primer 5 details |
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| Sequence | AGGGTCCTGAAATTCCGAGC | TGGAAGTAAACTAAACCTAGGTACGT | Start: 49 |
| Tm | 59.747 | 59.507 | End: 171 |
| Size | 20 | 26 | 123 |
