ssr_primer
Primer Details for TRINITY_DN81714_c1_g1_i2 |
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| (A)18tataagtagtt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCTCCACTGGACATCCAA | TGGGGGTTGGGCTACTTAGA | Start: 158 |
| Tm | 59.963 | 59.88 | End: 275 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
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| Sequence | ACCCGGACAATTGAAGGCAA | CCTTGGGGGTTGGGTTTCTT | Start: 61 |
| Tm | 60.179 | 60.105 | End: 199 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
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| Sequence | GTGCTCCACTGGACATCCAA | TGTGACATGAGGAGCAGCTC | Start: 158 |
| Tm | 59.963 | 59.751 | End: 296 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
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| Sequence | ACCCGGACAATTGAAGGCAA | TGGGGGTTGGGCTACTTAGA | Start: 61 |
| Tm | 60.179 | 59.88 | End: 275 |
| Size | 20 | 20 | 215 |
| Primer 5 details |
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| Sequence | AAGAAACCCAACCCCCAAGG | TGTGACATGAGGAGCAGCTC | Start: 180 |
| Tm | 60.105 | 59.751 | End: 296 |
| Size | 20 | 20 | 117 |
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