ssr_primer
Primer Details for TRINITY_DN81691_c0_g1_i3 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCTCTGTCTGCAAGCAT | GGCAGTTGGTTGCAGTTGAG | Start: 3053 |
| Tm | 59.964 | 59.969 | End: 3256 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
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| Sequence | GAAAGTGCTGGGCAAAGGTG | CTGCTTTGGTATGGGCGGTA | Start: 812 |
| Tm | 59.967 | 60.107 | End: 957 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
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| Sequence | GAAAGTGCTGGGCAAAGGTG | TCTCTGCTTTGGTATGGGCG | Start: 812 |
| Tm | 59.967 | 60.108 | End: 960 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
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| Sequence | ACTAGGCTCCTCTCCCGAAG | GACGGGGCAACATAGGTGAA | Start: 1535 |
| Tm | 60.106 | 60.036 | End: 1780 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
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| Sequence | AAGACTAGGCTCCTCTCCCG | GACGGGGCAACATAGGTGAA | Start: 1532 |
| Tm | 60.106 | 60.036 | End: 1780 |
| Size | 20 | 20 | 249 |
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