Primer Details for TRINITY_DN81653_c5_g1_i1 | |||
|---|---|---|---|
| (T)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCAGTTATTCCGATCGGGA | TCAGTAATTTGACTCAGACACTGT | Start: 53 |
| Tm | 59.968 | 57.838 | End: 179 |
| Size | 20 | 24 | 127 |
| Primer 2 details | |||
| Sequence | TCCCGCAGTTATTCCGATCG | TCAGTAATTTGACTCAGACACTGT | Start: 50 |
| Tm | 59.968 | 57.838 | End: 179 |
| Size | 20 | 24 | 130 |
| Primer 3 details | |||
| Sequence | TCCGATCGGGAGTGTAGGAA | TCAGTAATTTGACTCAGACACTGT | Start: 62 |
| Tm | 59.743 | 57.838 | End: 179 |
| Size | 20 | 24 | 118 |
| Primer 4 details | |||
| Sequence | CGCAGTTATTCCGATCGGGA | TCAGTAATTTGACTCAGACACTGTT | Start: 53 |
| Tm | 59.968 | 58.417 | End: 179 |
| Size | 20 | 25 | 127 |
| Primer 5 details | |||
| Sequence | CGCAGTTATTCCGATCGGGA | TTCAGTAATTTGACTCAGACACTGT | Start: 53 |
| Tm | 59.968 | 58.417 | End: 180 |
| Size | 20 | 25 | 128 |
