Primer Details for TRINITY_DN81649_c3_g3_i1 |
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| (A)12ttaaactataaagatctg(A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACGTGTGCTCTTCCGATT | TGGTTTAACTTTAGGAACGAAAAACCT | Start: 13 |
| Tm | 59.396 | 59.877 | End: 163 |
| Size | 20 | 27 | 151 |
| Primer 2 details |
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| Sequence | AGACGTGTGCTCTTCCGATT | TGGTTTAACTTTAGGAACGAAAAACC | Start: 13 |
| Tm | 59.396 | 58.743 | End: 163 |
| Size | 20 | 26 | 151 |
| Primer 3 details |
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| Sequence | AGACGTGTGCTCTTCCGATT | TGTTGATTGGTTTAACTTTAGGAACGA | Start: 13 |
| Tm | 59.396 | 59.717 | End: 170 |
| Size | 20 | 27 | 158 |
| Primer 4 details |
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| Sequence | AGACGTGTGCTCTTCCGATT | TGTTGATTGGTTTAACTTTAGGAACG | Start: 13 |
| Tm | 59.396 | 58.581 | End: 170 |
| Size | 20 | 26 | 158 |
| Primer 5 details |
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| Sequence | AGACGTGTGCTCTTCCGATT | GGTTTAACTTTAGGAACGAAAAACCT | Start: 13 |
| Tm | 59.396 | 58.517 | End: 162 |
| Size | 20 | 26 | 150 |
