ssr_primer
Primer Details for TRINITY_DN81594_c1_g1_i2 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGTGTCACAGGGAATCG | ACAAAGACGGGGCACAATGA | Start: 426 |
| Tm | 59.964 | 60.179 | End: 673 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
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| Sequence | TTGGGTGTCACAGGGAATCG | GAAGAGGAGGGCAATCGGAC | Start: 426 |
| Tm | 59.964 | 60.179 | End: 702 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
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| Sequence | GGGGAGGGTGGTGCAATTAA | ACAAAGACGGGGCACAATGA | Start: 472 |
| Tm | 59.96 | 60.179 | End: 673 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
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| Sequence | GGGGAGGGTGGTGCAATTAA | GAAGAGGAGGGCAATCGGAC | Start: 472 |
| Tm | 59.96 | 60.179 | End: 702 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
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| Sequence | TTGGGTGTCACAGGGAATCG | GACGAACGGATCTCATGGGG | Start: 426 |
| Tm | 59.964 | 60.249 | End: 651 |
| Size | 20 | 20 | 226 |
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