Primer Details for TRINITY_DN81526_c5_g4_i3 | |||
|---|---|---|---|
| (A)22: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACTTGCTGCCAACCTTAC | ACTCAAATGCACTATTACGACTGT | Start: 72 |
| Tm | 60.039 | 58.521 | End: 173 |
| Size | 20 | 24 | 102 |
| Primer 2 details | |||
| Sequence | TTTTTGTGGATGGGAGGGGG | ACTCAAATGCACTATTACGACTGT | Start: 19 |
| Tm | 59.884 | 58.521 | End: 173 |
| Size | 20 | 24 | 155 |
| Primer 3 details | |||
| Sequence | GCACTTGCTGCCAACCTTAC | ACTCAAATGCACTATTACGACTGTT | Start: 72 |
| Tm | 60.039 | 59.068 | End: 173 |
| Size | 20 | 25 | 102 |
| Primer 4 details | |||
| Sequence | TTTTTGTGGATGGGAGGGGG | ACTCAAATGCACTATTACGACTGTT | Start: 19 |
| Tm | 59.884 | 59.068 | End: 173 |
| Size | 20 | 25 | 155 |
| Primer 5 details | |||
| Sequence | ATAACCAACCGCACTTGCTG | ACTCAAATGCACTATTACGACTGT | Start: 62 |
| Tm | 59.119 | 58.521 | End: 173 |
| Size | 20 | 24 | 112 |
