ssr_primer
Primer Details for TRINITY_DN81501_c5_g4_i1 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGGACTGAATTCCCTCAA | TGCGGGTTTGCGGTAAAATG | Start: 545 |
| Tm | 60.035 | 60.039 | End: 733 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
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| Sequence | CGGAGGAACGTTTGCACAAG | TCGGCCTACTCTCGACTTGA | Start: 1365 |
| Tm | 60.041 | 60.036 | End: 1491 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
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| Sequence | GTGAGTCTCCCTTGTTGCCA | CTGCCTTGGACTCACGTCTT | Start: 364 |
| Tm | 59.891 | 59.966 | End: 515 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
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| Sequence | CTGCCGCCCACTATGAGAAA | ACACGCACATCCCTAAAGCA | Start: 1972 |
| Tm | 60.108 | 59.964 | End: 2106 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
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| Sequence | GTGAGTCTCCCTTGTTGCCA | TTGAGGGAATTCAGTCCGGC | Start: 364 |
| Tm | 59.891 | 60.035 | End: 564 |
| Size | 20 | 20 | 201 |
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