ssr_primer
Primer Details for TRINITY_DN81460_c3_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATTTTCCCCTCGCCTGA | ATCGCTCCGCTAACGTAAGG | Start: 270 |
| Tm | 60.034 | 59.97 | End: 396 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | CACTTTCCGCTGAACCAACG | TTTCCACGTGAGCCCTGTAC | Start: 96 |
| Tm | 60.041 | 59.966 | End: 365 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
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| Sequence | CACTTTCCGCTGAACCAACG | TCAGGCGAGGGGAAAATTCC | Start: 96 |
| Tm | 60.041 | 60.034 | End: 289 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
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| Sequence | CACTTTCCGCTGAACCAACG | AATTTCCACGTGAGCCCTGT | Start: 96 |
| Tm | 60.041 | 59.89 | End: 367 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
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| Sequence | CACTTTCCGCTGAACCAACG | CGCTTTTCGGAGCTCTTGTG | Start: 96 |
| Tm | 60.041 | 59.835 | End: 331 |
| Size | 20 | 20 | 236 |
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