ssr_primer
Primer Details for TRINITY_DN81447_c1_g1_i1 |
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| (AAAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGAGGAAATTCGGCAACT | TGGTGCTCATCTTCTTCGGG | Start: 125 |
| Tm | 59.676 | 59.75 | End: 363 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
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| Sequence | TCGGAGGAAATTCGGCAACT | GGTGCTCATCTTCTTCGGGG | Start: 125 |
| Tm | 59.676 | 60.463 | End: 362 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
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| Sequence | TCATACGGCGTGTTTCCTCA | TGGTGCTCATCTTCTTCGGG | Start: 154 |
| Tm | 59.396 | 59.75 | End: 363 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | TCGGAGGAAATTCGGCAACT | GTGCTCATCTTCTTCGGGGA | Start: 125 |
| Tm | 59.676 | 59.463 | End: 361 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | CGGAGGAAATTCGGCAACTC | TGGTGCTCATCTTCTTCGGG | Start: 126 |
| Tm | 59.273 | 59.75 | End: 363 |
| Size | 20 | 20 | 238 |
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