ssr_primer
Primer Details for TRINITY_DN81310_c1_g1_i1 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCTGGTCTAAAAGCGCT | CGAGCTTCGATTGCTTCAGC | Start: 576 |
| Tm | 60.035 | 59.974 | End: 798 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GCTGAAGCAATCGAAGCTCG | AGTGAGGAGCATTTGGTGGG | Start: 779 |
| Tm | 59.974 | 59.961 | End: 878 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | TCCCACCAAATGCTCCTCAC | GACAGCATCCTCTATCCGGC | Start: 858 |
| Tm | 59.962 | 60.038 | End: 1075 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | CCCACCAAATGCTCCTCACT | GACAGCATCCTCTATCCGGC | Start: 859 |
| Tm | 59.961 | 60.038 | End: 1075 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | CACTCCCACCAAATGCTCCT | GACAGCATCCTCTATCCGGC | Start: 855 |
| Tm | 59.961 | 60.038 | End: 1075 |
| Size | 20 | 20 | 221 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCTGGTCTAAAAGCGCT | CGAGCTTCGATTGCTTCAGC | Start: 576 |
| Tm | 60.035 | 59.974 | End: 798 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GCTGAAGCAATCGAAGCTCG | AGTGAGGAGCATTTGGTGGG | Start: 779 |
| Tm | 59.974 | 59.961 | End: 878 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | TCCCACCAAATGCTCCTCAC | GACAGCATCCTCTATCCGGC | Start: 858 |
| Tm | 59.962 | 60.038 | End: 1075 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | CCCACCAAATGCTCCTCACT | GACAGCATCCTCTATCCGGC | Start: 859 |
| Tm | 59.961 | 60.038 | End: 1075 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | CACTCCCACCAAATGCTCCT | GACAGCATCCTCTATCCGGC | Start: 855 |
| Tm | 59.961 | 60.038 | End: 1075 |
| Size | 20 | 20 | 221 |
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