ssr_primer
Primer Details for TRINITY_DN81305_c1_g1_i5 |
|---|
| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATTCTCGAGGCTTGCCTGT | TATCCCACAGAGGAGCAGCT | Start: 836 |
| Tm | 60.108 | 60.032 | End: 1061 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
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| Sequence | GATAGCACCACCCTACCACG | CGTCAGAGCCTCTATGAGCG | Start: 590 |
| Tm | 59.896 | 60.041 | End: 797 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | AGCTGCTCCTCTGTGGGATA | AGGGTTCTTTCTTGCCCCTG | Start: 1042 |
| Tm | 60.032 | 59.887 | End: 1212 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | TGCTGTCTCGGAGTCGGATA | CGTCAGAGCCTCTATGAGCG | Start: 658 |
| Tm | 60.107 | 60.041 | End: 797 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
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| Sequence | CCAACTCTGCTGTCTCGGAG | CGTCAGAGCCTCTATGAGCG | Start: 651 |
| Tm | 60.109 | 60.041 | End: 797 |
| Size | 20 | 20 | 147 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATTCTCGAGGCTTGCCTGT | TATCCCACAGAGGAGCAGCT | Start: 836 |
| Tm | 60.108 | 60.032 | End: 1061 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | GATAGCACCACCCTACCACG | CGTCAGAGCCTCTATGAGCG | Start: 590 |
| Tm | 59.896 | 60.041 | End: 797 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | AGCTGCTCCTCTGTGGGATA | AGGGTTCTTTCTTGCCCCTG | Start: 1042 |
| Tm | 60.032 | 59.887 | End: 1212 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | TGCTGTCTCGGAGTCGGATA | CGTCAGAGCCTCTATGAGCG | Start: 658 |
| Tm | 60.107 | 60.041 | End: 797 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | CCAACTCTGCTGTCTCGGAG | CGTCAGAGCCTCTATGAGCG | Start: 651 |
| Tm | 60.109 | 60.041 | End: 797 |
| Size | 20 | 20 | 147 |
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