ssr_primer
Primer Details for TRINITY_DN81254_c6_g1_i4 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCATTCGTTCGGGTGTGA | TCTCCTGCTTCGTTGGTGTC | Start: 17 |
| Tm | 60.038 | 59.966 | End: 181 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | TCGAACCGAATTTCCGAGCA | TCTCCTGCTTCGTTGGTGTC | Start: 59 |
| Tm | 60.038 | 59.966 | End: 181 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | TGGGGCGGCTTCAATAAACT | AAAGCGGTTGGAGCGTGATA | Start: 482 |
| Tm | 59.961 | 60.037 | End: 617 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | CTCTTGGGGCGGCTTCAATA | AAAGCGGTTGGAGCGTGATA | Start: 478 |
| Tm | 60.107 | 60.037 | End: 617 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | CGAGAGAACTCCCACCTTCG | AAAGCGGTTGGAGCGTGATA | Start: 517 |
| Tm | 59.829 | 60.037 | End: 617 |
| Size | 20 | 20 | 101 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCATTCGTTCGGGTGTGA | TCTCCTGCTTCGTTGGTGTC | Start: 17 |
| Tm | 60.038 | 59.966 | End: 181 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | TCGAACCGAATTTCCGAGCA | TCTCCTGCTTCGTTGGTGTC | Start: 59 |
| Tm | 60.038 | 59.966 | End: 181 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | TGGGGCGGCTTCAATAAACT | AAAGCGGTTGGAGCGTGATA | Start: 482 |
| Tm | 59.961 | 60.037 | End: 617 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | CTCTTGGGGCGGCTTCAATA | AAAGCGGTTGGAGCGTGATA | Start: 478 |
| Tm | 60.107 | 60.037 | End: 617 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | CGAGAGAACTCCCACCTTCG | AAAGCGGTTGGAGCGTGATA | Start: 517 |
| Tm | 59.829 | 60.037 | End: 617 |
| Size | 20 | 20 | 101 |
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