ssr_primer
Primer Details for TRINITY_DN81251_c3_g1_i7 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTGCGTAAAGGTCTTGG | CACTCCGCTTGAAAATGGGC | Start: 1229 |
| Tm | 59.964 | 60.109 | End: 1410 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | GAAGGCTGGCTCGTAGGTTT | CAAGGCGCATAGGTAGGTGT | Start: 3010 |
| Tm | 60.036 | 59.822 | End: 3198 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | GAAGGCTGGCTCGTAGGTTT | ACAAGGCGCATAGGTAGGTG | Start: 3010 |
| Tm | 60.036 | 59.822 | End: 3199 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | TTGCACTGGCTGTTAACCCT | AAACCTACGAGCCAGCCTTC | Start: 2921 |
| Tm | 59.815 | 60.036 | End: 3029 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | ACGGTGGCATAGTAACTGGC | CAAGGCGCATAGGTAGGTGT | Start: 2945 |
| Tm | 60.108 | 59.822 | End: 3198 |
| Size | 20 | 20 | 254 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTGCGTAAAGGTCTTGG | CACTCCGCTTGAAAATGGGC | Start: 1229 |
| Tm | 59.964 | 60.109 | End: 1410 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | GAAGGCTGGCTCGTAGGTTT | CAAGGCGCATAGGTAGGTGT | Start: 3010 |
| Tm | 60.036 | 59.822 | End: 3198 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | GAAGGCTGGCTCGTAGGTTT | ACAAGGCGCATAGGTAGGTG | Start: 3010 |
| Tm | 60.036 | 59.822 | End: 3199 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | TTGCACTGGCTGTTAACCCT | AAACCTACGAGCCAGCCTTC | Start: 2921 |
| Tm | 59.815 | 60.036 | End: 3029 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | ACGGTGGCATAGTAACTGGC | CAAGGCGCATAGGTAGGTGT | Start: 2945 |
| Tm | 60.108 | 59.822 | End: 3198 |
| Size | 20 | 20 | 254 |
(A)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTGCGTAAAGGTCTTGG | CACTCCGCTTGAAAATGGGC | Start: 1229 |
| Tm | 59.964 | 60.109 | End: 1410 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | GAAGGCTGGCTCGTAGGTTT | CAAGGCGCATAGGTAGGTGT | Start: 3010 |
| Tm | 60.036 | 59.822 | End: 3198 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | GAAGGCTGGCTCGTAGGTTT | ACAAGGCGCATAGGTAGGTG | Start: 3010 |
| Tm | 60.036 | 59.822 | End: 3199 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | TTGCACTGGCTGTTAACCCT | AAACCTACGAGCCAGCCTTC | Start: 2921 |
| Tm | 59.815 | 60.036 | End: 3029 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | ACGGTGGCATAGTAACTGGC | CAAGGCGCATAGGTAGGTGT | Start: 2945 |
| Tm | 60.108 | 59.822 | End: 3198 |
| Size | 20 | 20 | 254 |
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