ssr_primer
Primer Details for TRINITY_DN81224_c5_g1_i3 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTTCGGATCGCAATTCAC | GGCCAACCCTTACGAGTTCA | Start: 147 |
| Tm | 59.972 | 59.964 | End: 287 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
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| Sequence | AGGCATTGCTTTAAGGGGCT | GCATCGACATGCGCTAACAG | Start: 1070 |
| Tm | 59.958 | 60.043 | End: 1196 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
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| Sequence | AGGCTTCGGATCGCAATTCA | GGCCAACCCTTACGAGTTCA | Start: 146 |
| Tm | 60.108 | 59.964 | End: 287 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
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| Sequence | TTCACTGTGGAGTTCTGCCC | GGCCAACCCTTACGAGTTCA | Start: 162 |
| Tm | 59.891 | 59.964 | End: 287 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
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| Sequence | GGCTTCGGATCGCAATTCAC | GGGTTTTTCCGTTGTTGCCA | Start: 147 |
| Tm | 59.972 | 59.824 | End: 397 |
| Size | 20 | 20 | 251 |
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