ssr_primer
Primer Details for TRINITY_DN81213_c1_g3_i1 |
|---|
| (T)10actttcagcacaaaatactttcgacttgttgcattcaaaacatctgaaa(T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTGCCCCAGTATGTTTT | ATTTTGCTACGGTGGCCAGG | Start: 290 |
| Tm | 59.888 | 60.965 | End: 484 |
| Size | 20 | 20 | 195 |
| Primer 2 details |
|---|
| Sequence | AAATTACACGGTGCCCCAGT | ATTTTGCTACGGTGGCCAGG | Start: 283 |
| Tm | 59.888 | 60.965 | End: 484 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | TTACACGGTGCCCCAGTATG | ATTTTGCTACGGTGGCCAGG | Start: 286 |
| Tm | 59.749 | 60.965 | End: 484 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCAGGTGCCCATGAAGTCTT | GCCCGTGCCACTGGTAAAAA | Start: 140 |
| Tm | 59.961 | 61.457 | End: 418 |
| Size | 20 | 20 | 279 |
| Primer 5 details |
|---|
| Sequence | ACGGTGCCCCAGTATGTTTT | GCCCGTGCCACTGGTAAAAA | Start: 290 |
| Tm | 59.888 | 61.457 | End: 418 |
| Size | 20 | 20 | 129 |
(A)25cgaaatttgcattcctaataccactgtatcctctccgtggattgtgtaaggttgt(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTGCCCCAGTATGTTTT | ATTTTGCTACGGTGGCCAGG | Start: 290 |
| Tm | 59.888 | 60.965 | End: 484 |
| Size | 20 | 20 | 195 |
| Primer 2 details |
|---|
| Sequence | AAATTACACGGTGCCCCAGT | ATTTTGCTACGGTGGCCAGG | Start: 283 |
| Tm | 59.888 | 60.965 | End: 484 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | TTACACGGTGCCCCAGTATG | ATTTTGCTACGGTGGCCAGG | Start: 286 |
| Tm | 59.749 | 60.965 | End: 484 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCAGGTGCCCATGAAGTCTT | GCCCGTGCCACTGGTAAAAA | Start: 140 |
| Tm | 59.961 | 61.457 | End: 418 |
| Size | 20 | 20 | 279 |
| Primer 5 details |
|---|
| Sequence | ACGGTGCCCCAGTATGTTTT | GCCCGTGCCACTGGTAAAAA | Start: 290 |
| Tm | 59.888 | 61.457 | End: 418 |
| Size | 20 | 20 | 129 |
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