ssr_primer
Primer Details for TRINITY_DN81153_c3_g2_i2 |
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| (A)40: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTAGGCGTCGTAAAGGGCC | TGCGATCCATTTCGGGTGAA | Start: 335 |
| Tm | 60.179 | 60.036 | End: 484 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
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| Sequence | ATTAGGCGTCGTAAAGGGCC | GGTCTCGATAAGGGCCGTTT | Start: 335 |
| Tm | 60.179 | 59.823 | End: 451 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
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| Sequence | ATTAGGCGTCGTAAAGGGCC | GGGAGGAGGGGTCTCGATAA | Start: 335 |
| Tm | 60.179 | 59.812 | End: 460 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
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| Sequence | ATTAGGCGTCGTAAAGGGCC | AGGAGGGGTCTCGATAAGGG | Start: 335 |
| Tm | 60.179 | 59.811 | End: 457 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
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| Sequence | CTACCGAGGATTGTGGCGAT | TGCGATCCATTTCGGGTGAA | Start: 317 |
| Tm | 59.611 | 60.036 | End: 484 |
| Size | 20 | 20 | 168 |
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