ssr_primer
Primer Details for TRINITY_DN81115_c4_g1_i6 |
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| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGAGCTCTTGCGATGGCT | CCTTGTTTGCGGGAACAGTG | Start: 659 |
| Tm | 60.036 | 59.969 | End: 862 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
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| Sequence | TTCGTAACCCTTGCGACACA | GAGATGAAAGGGCCAGCCTT | Start: 1712 |
| Tm | 59.896 | 60.034 | End: 1970 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
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| Sequence | ACCTCGGCTGACTTCTGTTG | TGTGTCGCAAGGGTTACGAA | Start: 1549 |
| Tm | 59.966 | 59.896 | End: 1731 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
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| Sequence | TTCCAAGGACAAGGCCGTTT | CAACAGAAGTCAGCCGAGGT | Start: 1361 |
| Tm | 60.106 | 59.966 | End: 1568 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
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| Sequence | ACACAGCGTTCCAAGGACAA | CAACAGAAGTCAGCCGAGGT | Start: 1353 |
| Tm | 60.107 | 59.966 | End: 1568 |
| Size | 20 | 20 | 216 |
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