ssr_primer
Primer Details for TRINITY_DN81106_c3_g2_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATGACTGCAAATGGGGA | CTTCTTTCGCACAAACCCGG | Start: 29 |
| Tm | 59.591 | 60.04 | End: 140 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
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| Sequence | CACCATGACTGCAAATGGGG | CTTCTTTCGCACAAACCCGG | Start: 28 |
| Tm | 59.467 | 60.04 | End: 140 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | TGAGGCGGAGGGAGAAGAAA | TCATAGTTCGCGACTGGACG | Start: 388 |
| Tm | 60.544 | 59.902 | End: 604 |
| Size | 20 | 20 | 217 |
| Primer 4 details |
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| Sequence | TGAGGCGGAGGGAGAAGAAA | TGTGCGTGAAGTATCCCGAC | Start: 388 |
| Tm | 60.544 | 60.109 | End: 654 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
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| Sequence | TGAGGCGGAGGGAGAAGAAA | GTGCGTGAAGTATCCCGACT | Start: 388 |
| Tm | 60.544 | 59.827 | End: 653 |
| Size | 20 | 20 | 266 |
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