ssr_primer
Primer Details for TRINITY_DN81025_c1_g1_i5 |
|---|
| (TTC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACATTGCTGGTGACGTCA | GTTTGAAAGCCCGCGAAGTT | Start: 1605 |
| Tm | 59.894 | 59.97 | End: 1880 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | ACATTGCTGGTGACGTCACT | GTTTGAAAGCCCGCGAAGTT | Start: 1607 |
| Tm | 59.893 | 59.97 | End: 1880 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | TCGCCCGTAGATCTCCGTAT | TCGACAACGGGTAAAACGGT | Start: 944 |
| Tm | 59.964 | 59.896 | End: 1127 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | ACGCCAGAGTTCCGAAACAT | TGACGTCACCAGCAATGTGA | Start: 1511 |
| Tm | 59.965 | 59.894 | End: 1624 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | ACCGTTTTACCCGTTGTCGA | TAGCGTCCTCAAGCAACCTG | Start: 1108 |
| Tm | 59.896 | 60.037 | End: 1249 |
| Size | 20 | 20 | 142 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACATTGCTGGTGACGTCA | GTTTGAAAGCCCGCGAAGTT | Start: 1605 |
| Tm | 59.894 | 59.97 | End: 1880 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | ACATTGCTGGTGACGTCACT | GTTTGAAAGCCCGCGAAGTT | Start: 1607 |
| Tm | 59.893 | 59.97 | End: 1880 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | TCGCCCGTAGATCTCCGTAT | TCGACAACGGGTAAAACGGT | Start: 944 |
| Tm | 59.964 | 59.896 | End: 1127 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | ACGCCAGAGTTCCGAAACAT | TGACGTCACCAGCAATGTGA | Start: 1511 |
| Tm | 59.965 | 59.894 | End: 1624 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | ACCGTTTTACCCGTTGTCGA | TAGCGTCCTCAAGCAACCTG | Start: 1108 |
| Tm | 59.896 | 60.037 | End: 1249 |
| Size | 20 | 20 | 142 |
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