ssr_primer
Primer Details for TRINITY_DN81023_c3_g2_i1 |
|---|
| (T)28: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTCGTCCCTCAGCAGTA | CACTCCCGGCACAGTTTAGT | Start: 416 |
| Tm | 59.962 | 59.965 | End: 685 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
|---|
| Sequence | GCCACCATTGTGCTTCCATG | TACTGCTGAGGGACGACTGA | Start: 295 |
| Tm | 60.109 | 59.962 | End: 435 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | CGGGCGTTGTACGTTCAAAG | TACTGCTGAGGGACGACTGA | Start: 208 |
| Tm | 60.111 | 59.962 | End: 435 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | ACTAAACTGTGCCGGGAGTG | TCTTTGCCTTTTCCCTCCCC | Start: 666 |
| Tm | 59.965 | 59.886 | End: 844 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | ACTAAACTGTGCCGGGAGTG | CCTCCCCCTTTTTCTGGCTT | Start: 666 |
| Tm | 59.965 | 59.885 | End: 831 |
| Size | 20 | 20 | 166 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTCGTCCCTCAGCAGTA | CACTCCCGGCACAGTTTAGT | Start: 416 |
| Tm | 59.962 | 59.965 | End: 685 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
|---|
| Sequence | GCCACCATTGTGCTTCCATG | TACTGCTGAGGGACGACTGA | Start: 295 |
| Tm | 60.109 | 59.962 | End: 435 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | CGGGCGTTGTACGTTCAAAG | TACTGCTGAGGGACGACTGA | Start: 208 |
| Tm | 60.111 | 59.962 | End: 435 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | ACTAAACTGTGCCGGGAGTG | TCTTTGCCTTTTCCCTCCCC | Start: 666 |
| Tm | 59.965 | 59.886 | End: 844 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | ACTAAACTGTGCCGGGAGTG | CCTCCCCCTTTTTCTGGCTT | Start: 666 |
| Tm | 59.965 | 59.885 | End: 831 |
| Size | 20 | 20 | 166 |
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