ssr_primer
Primer Details for TRINITY_DN80975_c3_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGACAAGAGTGACGACTGC | ATATTCCGCCATGCCTACCG | Start: 1219 |
| Tm | 59.968 | 60.036 | End: 1392 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | TGGACAAGAGTGACGACTGC | TAAGCAGGCAGGATTCCACC | Start: 1219 |
| Tm | 59.968 | 59.744 | End: 1344 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | GGACAAGAGTGACGACTGCT | ATATTCCGCCATGCCTACCG | Start: 1220 |
| Tm | 59.684 | 60.036 | End: 1392 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TGGACAAGAGTGACGACTGC | GCAGGATTCCACCAGACAGT | Start: 1219 |
| Tm | 59.968 | 59.674 | End: 1337 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | CTTCGGACAAGGCAGCACTA | TCCGCTGCTCAGACATCAAA | Start: 2803 |
| Tm | 60.037 | 59.679 | End: 2959 |
| Size | 20 | 20 | 157 |
(G)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGACAAGAGTGACGACTGC | ATATTCCGCCATGCCTACCG | Start: 1219 |
| Tm | 59.968 | 60.036 | End: 1392 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | TGGACAAGAGTGACGACTGC | TAAGCAGGCAGGATTCCACC | Start: 1219 |
| Tm | 59.968 | 59.744 | End: 1344 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | GGACAAGAGTGACGACTGCT | ATATTCCGCCATGCCTACCG | Start: 1220 |
| Tm | 59.684 | 60.036 | End: 1392 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TGGACAAGAGTGACGACTGC | GCAGGATTCCACCAGACAGT | Start: 1219 |
| Tm | 59.968 | 59.674 | End: 1337 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | CTTCGGACAAGGCAGCACTA | TCCGCTGCTCAGACATCAAA | Start: 2803 |
| Tm | 60.037 | 59.679 | End: 2959 |
| Size | 20 | 20 | 157 |
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