ssr_primer
Primer Details for TRINITY_DN80922_c4_g1_i1 |
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| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATTCGACACTGGCCACTT | CCCAGATTCTCCCAAGTTCCA | Start: 247 |
| Tm | 60.322 | 59.365 | End: 454 |
| Size | 20 | 21 | 208 |
| Primer 2 details |
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| Sequence | AAGGATTCGACACTGGCCAC | CCCAGATTCTCCCAAGTTCCA | Start: 245 |
| Tm | 60.322 | 59.365 | End: 454 |
| Size | 20 | 21 | 210 |
| Primer 3 details |
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| Sequence | CGTTCGGGCTTTAATCCACG | CCCAGATTCTCCCAAGTTCCA | Start: 272 |
| Tm | 59.628 | 59.365 | End: 454 |
| Size | 20 | 21 | 183 |
| Primer 4 details |
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| Sequence | ACGTTCTTTCGTGAGGGAAC | GTGGCCAGTGTCGAATCCTT | Start: 84 |
| Tm | 58.138 | 60.322 | End: 264 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
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| Sequence | ACGTTCTTTCGTGAGGGAAC | AAGTGGCCAGTGTCGAATCC | Start: 84 |
| Tm | 58.138 | 60.322 | End: 266 |
| Size | 20 | 20 | 183 |
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