ssr_primer
Primer Details for TRINITY_DN80867_c1_g1_i3 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGACAAAGTGCATTGGCGG | TTGTTCGGATCGCCTCCAAA | Start: 1930 |
| Tm | 59.968 | 59.964 | End: 2181 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | GAAAAAGAGGGAGGCCACGA | CCGCCAATGCACTTTGTCAA | Start: 1840 |
| Tm | 59.964 | 59.968 | End: 1949 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | CGGTGATGAAGACTGCCTGT | GTGTAGCCTCCACCGAACAA | Start: 727 |
| Tm | 60.037 | 59.966 | End: 831 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | CCTGCCCGTGACTTAACACT | GGTACTTGGGGGTCCTGTTG | Start: 2420 |
| Tm | 59.965 | 59.962 | End: 2586 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | GAAGTCACTGAGGGCAAGCT | ACAGGCAGTCTTCATCACCG | Start: 512 |
| Tm | 59.964 | 60.037 | End: 746 |
| Size | 20 | 20 | 235 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGACAAAGTGCATTGGCGG | TTGTTCGGATCGCCTCCAAA | Start: 1930 |
| Tm | 59.968 | 59.964 | End: 2181 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | GAAAAAGAGGGAGGCCACGA | CCGCCAATGCACTTTGTCAA | Start: 1840 |
| Tm | 59.964 | 59.968 | End: 1949 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | CGGTGATGAAGACTGCCTGT | GTGTAGCCTCCACCGAACAA | Start: 727 |
| Tm | 60.037 | 59.966 | End: 831 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | CCTGCCCGTGACTTAACACT | GGTACTTGGGGGTCCTGTTG | Start: 2420 |
| Tm | 59.965 | 59.962 | End: 2586 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | GAAGTCACTGAGGGCAAGCT | ACAGGCAGTCTTCATCACCG | Start: 512 |
| Tm | 59.964 | 60.037 | End: 746 |
| Size | 20 | 20 | 235 |
(GAA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGACAAAGTGCATTGGCGG | TTGTTCGGATCGCCTCCAAA | Start: 1930 |
| Tm | 59.968 | 59.964 | End: 2181 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | GAAAAAGAGGGAGGCCACGA | CCGCCAATGCACTTTGTCAA | Start: 1840 |
| Tm | 59.964 | 59.968 | End: 1949 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | CGGTGATGAAGACTGCCTGT | GTGTAGCCTCCACCGAACAA | Start: 727 |
| Tm | 60.037 | 59.966 | End: 831 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | CCTGCCCGTGACTTAACACT | GGTACTTGGGGGTCCTGTTG | Start: 2420 |
| Tm | 59.965 | 59.962 | End: 2586 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | GAAGTCACTGAGGGCAAGCT | ACAGGCAGTCTTCATCACCG | Start: 512 |
| Tm | 59.964 | 60.037 | End: 746 |
| Size | 20 | 20 | 235 |
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