ssr_primer
Primer Details for TRINITY_DN80853_c3_g2_i2 |
|---|
| (GAA)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCCTCCCTTTTGTCACC | CCCAGCCGATTCACCCTATC | Start: 411 |
| Tm | 59.962 | 59.965 | End: 565 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | GATAGGGTGAATCGGCTGGG | AAACGGTGCGAACATGAAGC | Start: 546 |
| Tm | 59.965 | 60.04 | End: 788 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | AGTGATCGAAACAAGCCGGT | TTTCGCCGCTGAAAAACGAG | Start: 109 |
| Tm | 59.965 | 60.042 | End: 248 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GCTCAGCCACTGTTCGTAGT | TTTCGCCGCTGAAAAACGAG | Start: 92 |
| Tm | 60.038 | 60.042 | End: 248 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | CTCGTTTTTCAGCGGCGAAA | GGTGACAAAAGGGAGGGAGG | Start: 229 |
| Tm | 60.042 | 59.962 | End: 430 |
| Size | 20 | 20 | 202 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCCTCCCTTTTGTCACC | CCCAGCCGATTCACCCTATC | Start: 411 |
| Tm | 59.962 | 59.965 | End: 565 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | GATAGGGTGAATCGGCTGGG | AAACGGTGCGAACATGAAGC | Start: 546 |
| Tm | 59.965 | 60.04 | End: 788 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | AGTGATCGAAACAAGCCGGT | TTTCGCCGCTGAAAAACGAG | Start: 109 |
| Tm | 59.965 | 60.042 | End: 248 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GCTCAGCCACTGTTCGTAGT | TTTCGCCGCTGAAAAACGAG | Start: 92 |
| Tm | 60.038 | 60.042 | End: 248 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | CTCGTTTTTCAGCGGCGAAA | GGTGACAAAAGGGAGGGAGG | Start: 229 |
| Tm | 60.042 | 59.962 | End: 430 |
| Size | 20 | 20 | 202 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCCTCCCTTTTGTCACC | CCCAGCCGATTCACCCTATC | Start: 411 |
| Tm | 59.962 | 59.965 | End: 565 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | GATAGGGTGAATCGGCTGGG | AAACGGTGCGAACATGAAGC | Start: 546 |
| Tm | 59.965 | 60.04 | End: 788 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | AGTGATCGAAACAAGCCGGT | TTTCGCCGCTGAAAAACGAG | Start: 109 |
| Tm | 59.965 | 60.042 | End: 248 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GCTCAGCCACTGTTCGTAGT | TTTCGCCGCTGAAAAACGAG | Start: 92 |
| Tm | 60.038 | 60.042 | End: 248 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | CTCGTTTTTCAGCGGCGAAA | GGTGACAAAAGGGAGGGAGG | Start: 229 |
| Tm | 60.042 | 59.962 | End: 430 |
| Size | 20 | 20 | 202 |
(T)50: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCCTCCCTTTTGTCACC | CCCAGCCGATTCACCCTATC | Start: 411 |
| Tm | 59.962 | 59.965 | End: 565 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | GATAGGGTGAATCGGCTGGG | AAACGGTGCGAACATGAAGC | Start: 546 |
| Tm | 59.965 | 60.04 | End: 788 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | AGTGATCGAAACAAGCCGGT | TTTCGCCGCTGAAAAACGAG | Start: 109 |
| Tm | 59.965 | 60.042 | End: 248 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GCTCAGCCACTGTTCGTAGT | TTTCGCCGCTGAAAAACGAG | Start: 92 |
| Tm | 60.038 | 60.042 | End: 248 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | CTCGTTTTTCAGCGGCGAAA | GGTGACAAAAGGGAGGGAGG | Start: 229 |
| Tm | 60.042 | 59.962 | End: 430 |
| Size | 20 | 20 | 202 |
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