ssr_primer
Primer Details for TRINITY_DN80846_c8_g5_i1 |
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| (AT)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTCCTCGTGAAAGTGGC | TTGGCCGAAAAGTAGCACCT | Start: 598 |
| Tm | 59.966 | 59.89 | End: 703 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
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| Sequence | TCAACGTTTCATCCGGTCGA | GCCACTTTCACGAGGACTGA | Start: 420 |
| Tm | 59.685 | 59.966 | End: 617 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | TCAACGTTTCATCCGGTCGA | CCTGATCAACTCCGCCACTT | Start: 420 |
| Tm | 59.685 | 60.036 | End: 630 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
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| Sequence | TCAGTCCTCGTGAAAGTGGC | ATTGACTTTGACCCCCTCGC | Start: 598 |
| Tm | 59.966 | 60.323 | End: 827 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
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| Sequence | AAGTGGCGGAGTTGATCAGG | ATTGACTTTGACCCCCTCGC | Start: 611 |
| Tm | 60.036 | 60.323 | End: 827 |
| Size | 20 | 20 | 217 |
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