ssr_primer
Primer Details for TRINITY_DN80819_c1_g2_i1 |
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| (A)10tatgcttgaacaataatat(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCCAACTCCTGTTTTGCT | TATCTGACTTGGGGCTTCGC | Start: 2092 |
| Tm | 59.738 | 59.822 | End: 2286 |
| Size | 20 | 20 | 195 |
| Primer 2 details |
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| Sequence | GGAGAAGAGAGAAGGTCCGC | GCTCCAATCCGAAATGCGTC | Start: 1544 |
| Tm | 59.542 | 59.972 | End: 1705 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
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| Sequence | GGAGAAGAGAGAAGGTCCGC | TCCAATCCGAAATGCGTCCA | Start: 1544 |
| Tm | 59.542 | 60.036 | End: 1703 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
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| Sequence | GGAGAAGAGAGAAGGTCCGC | TTGCTCCAATCCGAAATGCG | Start: 1544 |
| Tm | 59.542 | 59.549 | End: 1707 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
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| Sequence | GGAGAAGAGAGAAGGTCCGC | CTCCAATCCGAAATGCGTCC | Start: 1544 |
| Tm | 59.542 | 59.342 | End: 1704 |
| Size | 20 | 20 | 161 |
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