ssr_primer
Primer Details for TRINITY_DN80732_c3_g4_i2 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCACAGGTGGTTCCTCTT | CCCTACTTGCATCATGGCGA | Start: 855 |
| Tm | 59.891 | 60.179 | End: 1112 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | CAGCACAGGTGGTTCCTCTT | CGGAAAGCGAAGGTGGATCA | Start: 855 |
| Tm | 59.891 | 60.391 | End: 1006 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CAGCACAGGTGGTTCCTCTT | ACGGAAAGCGAAGGTGGATC | Start: 855 |
| Tm | 59.891 | 60.391 | End: 1007 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | TGATCCACCTTCGCTTTCCG | CCCTACTTGCATCATGGCGA | Start: 987 |
| Tm | 60.391 | 60.179 | End: 1112 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | GATCCACCTTCGCTTTCCGT | CCCTACTTGCATCATGGCGA | Start: 988 |
| Tm | 60.391 | 60.179 | End: 1112 |
| Size | 20 | 20 | 125 |
(T)11gggatggatgttgtcccatcgacacggaaatcttcttttttttc(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCACAGGTGGTTCCTCTT | CCCTACTTGCATCATGGCGA | Start: 855 |
| Tm | 59.891 | 60.179 | End: 1112 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | CAGCACAGGTGGTTCCTCTT | CGGAAAGCGAAGGTGGATCA | Start: 855 |
| Tm | 59.891 | 60.391 | End: 1006 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CAGCACAGGTGGTTCCTCTT | ACGGAAAGCGAAGGTGGATC | Start: 855 |
| Tm | 59.891 | 60.391 | End: 1007 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | TGATCCACCTTCGCTTTCCG | CCCTACTTGCATCATGGCGA | Start: 987 |
| Tm | 60.391 | 60.179 | End: 1112 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | GATCCACCTTCGCTTTCCGT | CCCTACTTGCATCATGGCGA | Start: 988 |
| Tm | 60.391 | 60.179 | End: 1112 |
| Size | 20 | 20 | 125 |
(T)39: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCACAGGTGGTTCCTCTT | CCCTACTTGCATCATGGCGA | Start: 855 |
| Tm | 59.891 | 60.179 | End: 1112 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | CAGCACAGGTGGTTCCTCTT | CGGAAAGCGAAGGTGGATCA | Start: 855 |
| Tm | 59.891 | 60.391 | End: 1006 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CAGCACAGGTGGTTCCTCTT | ACGGAAAGCGAAGGTGGATC | Start: 855 |
| Tm | 59.891 | 60.391 | End: 1007 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | TGATCCACCTTCGCTTTCCG | CCCTACTTGCATCATGGCGA | Start: 987 |
| Tm | 60.391 | 60.179 | End: 1112 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | GATCCACCTTCGCTTTCCGT | CCCTACTTGCATCATGGCGA | Start: 988 |
| Tm | 60.391 | 60.179 | End: 1112 |
| Size | 20 | 20 | 125 |
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