ssr_primer
Primer Details for TRINITY_DN80708_c4_g8_i1 |
|---|
| (A)34: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACCGATGACAAGAGCGT | TGCGATTTTGCGGGAAGTTG | Start: 80 |
| Tm | 59.966 | 60.04 | End: 292 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | GACGCAGACCGCTAACGATA | ACCTCGCAAAACAAACAGCC | Start: 1017 |
| Tm | 59.971 | 59.898 | End: 1238 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | GACGCAGACCGCTAACGATA | GCTAGACACCCTACCGCTTC | Start: 1017 |
| Tm | 59.971 | 59.897 | End: 1273 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | TTTTCAAATTGGCCTGCCCG | AGTATCGCGACTCAAGCTGG | Start: 1535 |
| Tm | 59.965 | 59.899 | End: 1709 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
|---|
| Sequence | GGCTGTTTGTTTTGCGAGGT | CGCTCAAGGTGTCCCTGATT | Start: 1219 |
| Tm | 59.898 | 60.036 | End: 1443 |
| Size | 20 | 20 | 225 |
(T)13attta(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACCGATGACAAGAGCGT | TGCGATTTTGCGGGAAGTTG | Start: 80 |
| Tm | 59.966 | 60.04 | End: 292 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | GACGCAGACCGCTAACGATA | ACCTCGCAAAACAAACAGCC | Start: 1017 |
| Tm | 59.971 | 59.898 | End: 1238 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | GACGCAGACCGCTAACGATA | GCTAGACACCCTACCGCTTC | Start: 1017 |
| Tm | 59.971 | 59.897 | End: 1273 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | TTTTCAAATTGGCCTGCCCG | AGTATCGCGACTCAAGCTGG | Start: 1535 |
| Tm | 59.965 | 59.899 | End: 1709 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
|---|
| Sequence | GGCTGTTTGTTTTGCGAGGT | CGCTCAAGGTGTCCCTGATT | Start: 1219 |
| Tm | 59.898 | 60.036 | End: 1443 |
| Size | 20 | 20 | 225 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACCGATGACAAGAGCGT | TGCGATTTTGCGGGAAGTTG | Start: 80 |
| Tm | 59.966 | 60.04 | End: 292 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | GACGCAGACCGCTAACGATA | ACCTCGCAAAACAAACAGCC | Start: 1017 |
| Tm | 59.971 | 59.898 | End: 1238 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | GACGCAGACCGCTAACGATA | GCTAGACACCCTACCGCTTC | Start: 1017 |
| Tm | 59.971 | 59.897 | End: 1273 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | TTTTCAAATTGGCCTGCCCG | AGTATCGCGACTCAAGCTGG | Start: 1535 |
| Tm | 59.965 | 59.899 | End: 1709 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
|---|
| Sequence | GGCTGTTTGTTTTGCGAGGT | CGCTCAAGGTGTCCCTGATT | Start: 1219 |
| Tm | 59.898 | 60.036 | End: 1443 |
| Size | 20 | 20 | 225 |
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