Primer Details for TRINITY_DN80707_c5_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCTTGTGCAATATCTTTTACTCA | TGTATTTCCCAACGTTACCATGT | Start: 5 |
| Tm | 57.855 | 58.346 | End: 199 |
| Size | 25 | 23 | 195 |
| Primer 2 details |
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| Sequence | CACCTTGTGCAATATCTTTTACTCA | TTGTATTTCCCAACGTTACCATGT | Start: 5 |
| Tm | 57.855 | 58.929 | End: 200 |
| Size | 25 | 24 | 196 |
| Primer 3 details |
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| Sequence | CACCTTGTGCAATATCTTTTACTCAA | TGTATTTCCCAACGTTACCATGT | Start: 5 |
| Tm | 58.407 | 58.346 | End: 199 |
| Size | 26 | 23 | 195 |
| Primer 4 details |
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| Sequence | ACCTTGTGCAATATCTTTTACTCAA | TGTATTTCCCAACGTTACCATGT | Start: 6 |
| Tm | 57.241 | 58.346 | End: 199 |
| Size | 25 | 23 | 194 |
| Primer 5 details |
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| Sequence | CACCTTGTGCAATATCTTTTACTCAA | TTGTATTTCCCAACGTTACCATGT | Start: 5 |
| Tm | 58.407 | 58.929 | End: 200 |
| Size | 26 | 24 | 196 |
