ssr_primer
Primer Details for TRINITY_DN80698_c3_g1_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACGACACACCACACAGTA | GCTAGGACGTTTCGACGAGT | Start: 1197 |
| Tm | 59.897 | 59.832 | End: 1446 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | CGGAAATGCTGTACGTCCCT | CTATTCTGCCGTGCGAAGGA | Start: 56 |
| Tm | 60.108 | 60.179 | End: 287 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | AAGGAGGTAGGACAAGGGCA | GGCTGGACTATGCCCCTTAC | Start: 468 |
| Tm | 60.179 | 59.891 | End: 674 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | TTAAGCCACGACACACCACA | GCTAGGACGTTTCGACGAGT | Start: 1192 |
| Tm | 59.822 | 59.832 | End: 1446 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | CAAGCCATCAAGCTGCAGAC | ATGTTCTGATCGGCTGCCAT | Start: 2914 |
| Tm | 59.829 | 59.818 | End: 3015 |
| Size | 20 | 20 | 102 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACGACACACCACACAGTA | GCTAGGACGTTTCGACGAGT | Start: 1197 |
| Tm | 59.897 | 59.832 | End: 1446 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | CGGAAATGCTGTACGTCCCT | CTATTCTGCCGTGCGAAGGA | Start: 56 |
| Tm | 60.108 | 60.179 | End: 287 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | AAGGAGGTAGGACAAGGGCA | GGCTGGACTATGCCCCTTAC | Start: 468 |
| Tm | 60.179 | 59.891 | End: 674 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | TTAAGCCACGACACACCACA | GCTAGGACGTTTCGACGAGT | Start: 1192 |
| Tm | 59.822 | 59.832 | End: 1446 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | CAAGCCATCAAGCTGCAGAC | ATGTTCTGATCGGCTGCCAT | Start: 2914 |
| Tm | 59.829 | 59.818 | End: 3015 |
| Size | 20 | 20 | 102 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACGACACACCACACAGTA | GCTAGGACGTTTCGACGAGT | Start: 1197 |
| Tm | 59.897 | 59.832 | End: 1446 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | CGGAAATGCTGTACGTCCCT | CTATTCTGCCGTGCGAAGGA | Start: 56 |
| Tm | 60.108 | 60.179 | End: 287 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | AAGGAGGTAGGACAAGGGCA | GGCTGGACTATGCCCCTTAC | Start: 468 |
| Tm | 60.179 | 59.891 | End: 674 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | TTAAGCCACGACACACCACA | GCTAGGACGTTTCGACGAGT | Start: 1192 |
| Tm | 59.822 | 59.832 | End: 1446 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | CAAGCCATCAAGCTGCAGAC | ATGTTCTGATCGGCTGCCAT | Start: 2914 |
| Tm | 59.829 | 59.818 | End: 3015 |
| Size | 20 | 20 | 102 |
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