ssr_primer
Primer Details for TRINITY_DN80626_c11_g1_i1 |
|---|
| (A)12g(A)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGTCGAGAAAAGGCCGTG | CCTTCCTTATCATTTTGGGAGTCG | Start: 10 |
| Tm | 59.765 | 59.424 | End: 234 |
| Size | 20 | 24 | 225 |
| Primer 2 details |
|---|
| Sequence | TCGTTGTCGAGAAAAGGCCG | CCTTCCTTATCATTTTGGGAGTCG | Start: 8 |
| Tm | 60.942 | 59.424 | End: 234 |
| Size | 20 | 24 | 227 |
| Primer 3 details |
|---|
| Sequence | TCGAGAAAAGGCCGTGCAT | CCTTCCTTATCATTTTGGGAGTCG | Start: 14 |
| Tm | 60.004 | 59.424 | End: 234 |
| Size | 19 | 24 | 221 |
| Primer 4 details |
|---|
| Sequence | GTTGTCGAGAAAAGGCCGTG | CTTCCTTATCATTTTGGGAGTCGA | Start: 10 |
| Tm | 59.765 | 58.572 | End: 233 |
| Size | 20 | 24 | 224 |
| Primer 5 details |
|---|
| Sequence | GTTGTCGAGAAAAGGCCGTG | TCCTTATCATTTTGGGAGTCGAA | Start: 10 |
| Tm | 59.765 | 57.57 | End: 231 |
| Size | 20 | 23 | 222 |
(T)12cgactcccaaaatgataaggaagg(A)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGTCGAGAAAAGGCCGTG | CCTTCCTTATCATTTTGGGAGTCG | Start: 10 |
| Tm | 59.765 | 59.424 | End: 234 |
| Size | 20 | 24 | 225 |
| Primer 2 details |
|---|
| Sequence | TCGTTGTCGAGAAAAGGCCG | CCTTCCTTATCATTTTGGGAGTCG | Start: 8 |
| Tm | 60.942 | 59.424 | End: 234 |
| Size | 20 | 24 | 227 |
| Primer 3 details |
|---|
| Sequence | TCGAGAAAAGGCCGTGCAT | CCTTCCTTATCATTTTGGGAGTCG | Start: 14 |
| Tm | 60.004 | 59.424 | End: 234 |
| Size | 19 | 24 | 221 |
| Primer 4 details |
|---|
| Sequence | GTTGTCGAGAAAAGGCCGTG | CTTCCTTATCATTTTGGGAGTCGA | Start: 10 |
| Tm | 59.765 | 58.572 | End: 233 |
| Size | 20 | 24 | 224 |
| Primer 5 details |
|---|
| Sequence | GTTGTCGAGAAAAGGCCGTG | TCCTTATCATTTTGGGAGTCGAA | Start: 10 |
| Tm | 59.765 | 57.57 | End: 231 |
| Size | 20 | 23 | 222 |
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