ssr_primer
Primer Details for TRINITY_DN80578_c1_g1_i2 |
|---|
| (T)10c(T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTCCCCTTCGCTGTTTG | GCGAGATCCTGAGGCTCAAA | Start: 2 |
| Tm | 60.039 | 59.823 | End: 281 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | TGCGCTACTGTGAGCTTACC | Start: 279 |
| Tm | 60.179 | 60.109 | End: 548 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | GACTGCGCTACTGTGAGCTT | Start: 279 |
| Tm | 60.179 | 60.389 | End: 551 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | AAGACTGCGCTACTGTGAGC | Start: 279 |
| Tm | 60.179 | 60.389 | End: 553 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | CGCTACTGTGAGCTTACCGG | Start: 279 |
| Tm | 60.179 | 60.526 | End: 546 |
| Size | 20 | 20 | 268 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTCCCCTTCGCTGTTTG | GCGAGATCCTGAGGCTCAAA | Start: 2 |
| Tm | 60.039 | 59.823 | End: 281 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | TGCGCTACTGTGAGCTTACC | Start: 279 |
| Tm | 60.179 | 60.109 | End: 548 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | GACTGCGCTACTGTGAGCTT | Start: 279 |
| Tm | 60.179 | 60.389 | End: 551 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | AAGACTGCGCTACTGTGAGC | Start: 279 |
| Tm | 60.179 | 60.389 | End: 553 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | CGCTACTGTGAGCTTACCGG | Start: 279 |
| Tm | 60.179 | 60.526 | End: 546 |
| Size | 20 | 20 | 268 |
(G)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTCCCCTTCGCTGTTTG | GCGAGATCCTGAGGCTCAAA | Start: 2 |
| Tm | 60.039 | 59.823 | End: 281 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | TGCGCTACTGTGAGCTTACC | Start: 279 |
| Tm | 60.179 | 60.109 | End: 548 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | GACTGCGCTACTGTGAGCTT | Start: 279 |
| Tm | 60.179 | 60.389 | End: 551 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | AAGACTGCGCTACTGTGAGC | Start: 279 |
| Tm | 60.179 | 60.389 | End: 553 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | CGCATCCTCATCGTCCCTTT | CGCTACTGTGAGCTTACCGG | Start: 279 |
| Tm | 60.179 | 60.526 | End: 546 |
| Size | 20 | 20 | 268 |
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