ssr_primer
Primer Details for TRINITY_DN80561_c4_g3_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAGTTCAGTCGGGAGCTT | GTCTGACACGGCGGATACAG | Start: 383 |
| Tm | 59.966 | 60.526 | End: 523 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | CAGTCGGGAGCTTAAAGGCA | GTCTGACACGGCGGATACAG | Start: 390 |
| Tm | 60.036 | 60.526 | End: 523 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | TGCATGATACAACTGGCCCA | CCTCCTGAGCAAAATGGGCT | Start: 1150 |
| Tm | 59.669 | 60.324 | End: 1312 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | TGCATGATACAACTGGCCCA | CCTTCCAACCTCCTGAGCAA | Start: 1150 |
| Tm | 59.669 | 59.598 | End: 1320 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | TCAGTCGGGAGCTTAAAGGC | GTCTGACACGGCGGATACAG | Start: 389 |
| Tm | 59.751 | 60.526 | End: 523 |
| Size | 20 | 20 | 135 |
(A)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAGTTCAGTCGGGAGCTT | GTCTGACACGGCGGATACAG | Start: 383 |
| Tm | 59.966 | 60.526 | End: 523 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | CAGTCGGGAGCTTAAAGGCA | GTCTGACACGGCGGATACAG | Start: 390 |
| Tm | 60.036 | 60.526 | End: 523 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | TGCATGATACAACTGGCCCA | CCTCCTGAGCAAAATGGGCT | Start: 1150 |
| Tm | 59.669 | 60.324 | End: 1312 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | TGCATGATACAACTGGCCCA | CCTTCCAACCTCCTGAGCAA | Start: 1150 |
| Tm | 59.669 | 59.598 | End: 1320 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | TCAGTCGGGAGCTTAAAGGC | GTCTGACACGGCGGATACAG | Start: 389 |
| Tm | 59.751 | 60.526 | End: 523 |
| Size | 20 | 20 | 135 |
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