ssr_primer
Primer Details for TRINITY_DN80541_c5_g1_i5 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCACCGCTGTCTTCATCT | AAGGACCCCTCACACTCCTT | Start: 2646 |
| Tm | 59.965 | 60.104 | End: 2833 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
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| Sequence | GCTGAAAGTTGGCGCAATGA | AAGGACCCCTCACACTCCTT | Start: 2718 |
| Tm | 60.039 | 60.104 | End: 2833 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
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| Sequence | CAAAGTCAGAATGTGCGGCC | ATCACGGCACACATTCCCAT | Start: 2153 |
| Tm | 60.11 | 60.034 | End: 2286 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | CCGCTTTTCCCTCCCTCAAT | GTACTTGACTCGGTTCGCGA | Start: 497 |
| Tm | 60.034 | 60.11 | End: 681 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
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| Sequence | ACCACTTGCTTGCAGGAGTT | TCATTGCGCCAACTTTCAGC | Start: 2614 |
| Tm | 60.107 | 60.039 | End: 2737 |
| Size | 20 | 20 | 124 |
(T)11caccaagaaattggaacttttgtacatcattaggcaatgtacttatggtacttatgccgcgattttaacaatg(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCACCGCTGTCTTCATCT | AAGGACCCCTCACACTCCTT | Start: 2646 |
| Tm | 59.965 | 60.104 | End: 2833 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | GCTGAAAGTTGGCGCAATGA | AAGGACCCCTCACACTCCTT | Start: 2718 |
| Tm | 60.039 | 60.104 | End: 2833 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | CAAAGTCAGAATGTGCGGCC | ATCACGGCACACATTCCCAT | Start: 2153 |
| Tm | 60.11 | 60.034 | End: 2286 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | CCGCTTTTCCCTCCCTCAAT | GTACTTGACTCGGTTCGCGA | Start: 497 |
| Tm | 60.034 | 60.11 | End: 681 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | ACCACTTGCTTGCAGGAGTT | TCATTGCGCCAACTTTCAGC | Start: 2614 |
| Tm | 60.107 | 60.039 | End: 2737 |
| Size | 20 | 20 | 124 |
ICAR Data Use Licence
