ssr_primer
Primer Details for TRINITY_DN80513_c6_g1_i2 |
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| (A)16tgatcttactgattgaaaaattaaattgttacgaaaaagtggccgatatgtttcaatgcattcatgt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCCACGCCACTGAGATAG | TAATGCTGTTTAGGGGCCGG | Start: 160 |
| Tm | 59.894 | 60.107 | End: 371 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
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| Sequence | CATGCCACGCCACTGAGATA | TAATGCTGTTTAGGGGCCGG | Start: 159 |
| Tm | 60.179 | 60.107 | End: 371 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
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| Sequence | GCCACGCCACTGAGATAGAG | TAATGCTGTTTAGGGGCCGG | Start: 162 |
| Tm | 60.249 | 60.107 | End: 371 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
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| Sequence | ATGCCACGCCACTGAGATAG | TGATGAAACAAACGCCTGGC | Start: 160 |
| Tm | 59.894 | 59.686 | End: 264 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
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| Sequence | GCCAGGCGTTTGTTTCATCA | TAATGCTGTTTAGGGGCCGG | Start: 245 |
| Tm | 59.686 | 60.107 | End: 371 |
| Size | 20 | 20 | 127 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCCACGCCACTGAGATAG | TAATGCTGTTTAGGGGCCGG | Start: 160 |
| Tm | 59.894 | 60.107 | End: 371 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | CATGCCACGCCACTGAGATA | TAATGCTGTTTAGGGGCCGG | Start: 159 |
| Tm | 60.179 | 60.107 | End: 371 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | GCCACGCCACTGAGATAGAG | TAATGCTGTTTAGGGGCCGG | Start: 162 |
| Tm | 60.249 | 60.107 | End: 371 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | ATGCCACGCCACTGAGATAG | TGATGAAACAAACGCCTGGC | Start: 160 |
| Tm | 59.894 | 59.686 | End: 264 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | GCCAGGCGTTTGTTTCATCA | TAATGCTGTTTAGGGGCCGG | Start: 245 |
| Tm | 59.686 | 60.107 | End: 371 |
| Size | 20 | 20 | 127 |
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