Primer Details for TRINITY_DN80486_c2_g6_i1 | |||
|---|---|---|---|
| (A)14t(A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCAGCAGTAAAAGTTTAGACCA | TCTCACCCGTTTGATGTATCGA | Start: 249 |
| Tm | 58.285 | 59.244 | End: 454 |
| Size | 23 | 22 | 206 |
| Primer 2 details | |||
| Sequence | GCAGCAGTAAAAGTTTAGACCATGT | TCTCACCCGTTTGATGTATCGA | Start: 250 |
| Tm | 60.049 | 59.244 | End: 454 |
| Size | 25 | 22 | 205 |
| Primer 3 details | |||
| Sequence | AGCAGCAGTAAAAGTTTAGACCA | ACAATCTCACCCGTTTGATGT | Start: 249 |
| Tm | 58.285 | 57.86 | End: 458 |
| Size | 23 | 21 | 210 |
| Primer 4 details | |||
| Sequence | AGCAGCAGTAAAAGTTTAGACCA | CTCACCCGTTTGATGTATCGA | Start: 249 |
| Tm | 58.285 | 57.841 | End: 453 |
| Size | 23 | 21 | 205 |
| Primer 5 details | |||
| Sequence | AGCAGCAGTAAAAGTTTAGACCA | TCTCACCCGTTTGATGTATCG | Start: 249 |
| Tm | 58.285 | 57.841 | End: 454 |
| Size | 23 | 21 | 206 |
