ssr_primer
Primer Details for TRINITY_DN80466_c5_g1_i1 |
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| (AATAA)4(AATAAAAAAAAAAATTGATACCTACAGACCAGAATTAA)(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTATCGCAAAACACGCCAC | TCCATGCTGAGTCGAAGCTT | Start: 21 |
| Tm | 59.764 | 59.39 | End: 122 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
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| Sequence | TGGAAAGCTTCGACTCAGCA | ACGCTGAAACGAGATCGAGT | Start: 99 |
| Tm | 59.607 | 59.478 | End: 301 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
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| Sequence | TGTATCGCAAAACACGCCAC | ATCCATGCTGAGTCGAAGCT | Start: 21 |
| Tm | 59.764 | 59.171 | End: 123 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
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| Sequence | GCCACTGCTGTCGTTGAATT | ACGCTGAAACGAGATCGAGT | Start: 41 |
| Tm | 59.407 | 59.478 | End: 301 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
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| Sequence | AAGCTTCGACTCAGCATGGA | ACGCTGAAACGAGATCGAGT | Start: 103 |
| Tm | 59.39 | 59.478 | End: 301 |
| Size | 20 | 20 | 199 |
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