ssr_primer
Primer Details for TRINITY_DN80325_c4_g1_i4 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCTCGTTGTGAGTGCAGA | GCACTCGGTCTCAGGGAAAA | Start: 1639 |
| Tm | 59.968 | 59.965 | End: 1803 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
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| Sequence | GGTCTCGTTGTGAGTGCAGA | AATGTTGGATGGCACTCGGT | Start: 1639 |
| Tm | 59.968 | 59.962 | End: 1814 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
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| Sequence | GCGGCAAAGACTCCCTAGTT | GCCTCCATGCAGACAACTCT | Start: 314 |
| Tm | 60.036 | 60.036 | End: 415 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
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| Sequence | GGTCTCGTTGTGAGTGCAGA | CCAAGGGCCCAGAGTTGAAT | Start: 1639 |
| Tm | 59.968 | 59.96 | End: 1901 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
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| Sequence | ACACTGCTCCATTCCGTGAG | ACAGGAACCCACATACGCAG | Start: 743 |
| Tm | 60.037 | 60.036 | End: 843 |
| Size | 20 | 20 | 101 |
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