ssr_primer
Primer Details for TRINITY_DN80320_c4_g2_i2 |
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| (A)39: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGCGCAACAGAAAAGTCC | GGTGGGAGAGTGAGGGTTTG | Start: 179 |
| Tm | 59.971 | 59.963 | End: 342 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
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| Sequence | AACGCGCAACAGAAAAGTCC | GGGAGAGTGAGGGTTTGACG | Start: 179 |
| Tm | 59.971 | 60.037 | End: 339 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
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| Sequence | CGCAACAGAAAAGTCCAGCC | GGTGGGAGAGTGAGGGTTTG | Start: 183 |
| Tm | 60.04 | 59.963 | End: 342 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
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| Sequence | CGCAACAGAAAAGTCCAGCC | GGGAGAGTGAGGGTTTGACG | Start: 183 |
| Tm | 60.04 | 60.037 | End: 339 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
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| Sequence | AACGCGCAACAGAAAAGTCC | AACCTGTCTGTGCTTGGCTT | Start: 179 |
| Tm | 59.971 | 60.107 | End: 310 |
| Size | 20 | 20 | 132 |
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