ssr_primer
Primer Details for TRINITY_DN80303_c2_g1_i1 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAACGGCAAAATGTCACCT | AGATAATCGGGGCATCTGCG | Start: 2359 |
| Tm | 59.968 | 60.037 | End: 2606 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
|---|
| Sequence | CTGGAGGCCTACCAAAAGCA | ACGTGTGTGGCTTATCTGCA | Start: 3063 |
| Tm | 59.962 | 59.965 | End: 3195 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | CGCAGATGCCCCGATTATCT | CTCGTCTGAACCAGTGGTCC | Start: 2587 |
| Tm | 60.037 | 60.038 | End: 2840 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | CGCAGATGCCCCGATTATCT | CCTCGTCTGAACCAGTGGTC | Start: 2587 |
| Tm | 60.037 | 60.038 | End: 2841 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | GACCACTGGTTCAGACGAGG | TGCTTTTGGTAGGCCTCCAG | Start: 2822 |
| Tm | 60.038 | 59.962 | End: 3082 |
| Size | 20 | 20 | 261 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAACGGCAAAATGTCACCT | AGATAATCGGGGCATCTGCG | Start: 2359 |
| Tm | 59.968 | 60.037 | End: 2606 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
|---|
| Sequence | CTGGAGGCCTACCAAAAGCA | ACGTGTGTGGCTTATCTGCA | Start: 3063 |
| Tm | 59.962 | 59.965 | End: 3195 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | CGCAGATGCCCCGATTATCT | CTCGTCTGAACCAGTGGTCC | Start: 2587 |
| Tm | 60.037 | 60.038 | End: 2840 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | CGCAGATGCCCCGATTATCT | CCTCGTCTGAACCAGTGGTC | Start: 2587 |
| Tm | 60.037 | 60.038 | End: 2841 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | GACCACTGGTTCAGACGAGG | TGCTTTTGGTAGGCCTCCAG | Start: 2822 |
| Tm | 60.038 | 59.962 | End: 3082 |
| Size | 20 | 20 | 261 |
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