ssr_primer
Primer Details for TRINITY_DN80226_c2_g2_i3 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGCAAAGAGTTGTGTTGA | GGTGCTTCAGCGTTAGCCTA | Start: 471 |
| Tm | 58.909 | 60.108 | End: 683 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | TATCCCATGGATGCAGCCAC | TCAACACAACTCTTTGCGCA | Start: 328 |
| Tm | 59.889 | 58.909 | End: 490 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | AGTGCGCAAAGAGTTGTGTT | GGTGCTTCAGCGTTAGCCTA | Start: 469 |
| Tm | 58.905 | 60.108 | End: 683 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | AAGTGCGCAAAGAGTTGTGT | GGTGCTTCAGCGTTAGCCTA | Start: 468 |
| Tm | 58.905 | 60.108 | End: 683 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TATCCCATGGATGCAGCCAC | ACACAACTCTTTGCGCACTT | Start: 328 |
| Tm | 59.889 | 58.905 | End: 487 |
| Size | 20 | 20 | 160 |
(T)24acaattgactgtcctcag(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGCAAAGAGTTGTGTTGA | GGTGCTTCAGCGTTAGCCTA | Start: 471 |
| Tm | 58.909 | 60.108 | End: 683 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | TATCCCATGGATGCAGCCAC | TCAACACAACTCTTTGCGCA | Start: 328 |
| Tm | 59.889 | 58.909 | End: 490 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | AGTGCGCAAAGAGTTGTGTT | GGTGCTTCAGCGTTAGCCTA | Start: 469 |
| Tm | 58.905 | 60.108 | End: 683 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | AAGTGCGCAAAGAGTTGTGT | GGTGCTTCAGCGTTAGCCTA | Start: 468 |
| Tm | 58.905 | 60.108 | End: 683 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TATCCCATGGATGCAGCCAC | ACACAACTCTTTGCGCACTT | Start: 328 |
| Tm | 59.889 | 58.905 | End: 487 |
| Size | 20 | 20 | 160 |
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