ssr_primer
Primer Details for TRINITY_DN80218_c0_g1_i9 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGCTAGATCGGCTCGGTG | CCACTTCCGGAGACCAAGAC | Start: 2250 |
| Tm | 59.897 | 60.037 | End: 2378 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | CGCCACTTATCAGGATGCCT | TAATCGTGTGGGAAGGCACC | Start: 2556 |
| Tm | 59.893 | 60.036 | End: 2687 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CGATACACCTTCTGCACCGT | TAATCGTGTGGGAAGGCACC | Start: 2507 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | GCTCCTCGTCATTGAACCGA | TAATCGTGTGGGAAGGCACC | Start: 2490 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | TCGCTCCTCGTCATTGAACC | TAATCGTGTGGGAAGGCACC | Start: 2488 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 200 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGCTAGATCGGCTCGGTG | CCACTTCCGGAGACCAAGAC | Start: 2250 |
| Tm | 59.897 | 60.037 | End: 2378 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | CGCCACTTATCAGGATGCCT | TAATCGTGTGGGAAGGCACC | Start: 2556 |
| Tm | 59.893 | 60.036 | End: 2687 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CGATACACCTTCTGCACCGT | TAATCGTGTGGGAAGGCACC | Start: 2507 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | GCTCCTCGTCATTGAACCGA | TAATCGTGTGGGAAGGCACC | Start: 2490 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | TCGCTCCTCGTCATTGAACC | TAATCGTGTGGGAAGGCACC | Start: 2488 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 200 |
(T)11a(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGCTAGATCGGCTCGGTG | CCACTTCCGGAGACCAAGAC | Start: 2250 |
| Tm | 59.897 | 60.037 | End: 2378 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | CGCCACTTATCAGGATGCCT | TAATCGTGTGGGAAGGCACC | Start: 2556 |
| Tm | 59.893 | 60.036 | End: 2687 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CGATACACCTTCTGCACCGT | TAATCGTGTGGGAAGGCACC | Start: 2507 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | GCTCCTCGTCATTGAACCGA | TAATCGTGTGGGAAGGCACC | Start: 2490 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | TCGCTCCTCGTCATTGAACC | TAATCGTGTGGGAAGGCACC | Start: 2488 |
| Tm | 60.109 | 60.036 | End: 2687 |
| Size | 20 | 20 | 200 |
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