Primer Details for TRINITY_DN80153_c6_g7_i1 |
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| (A)27: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATTCTAAAATTGCCTGTATCTTTGT | CAGCACTTGCAGCAGAAATGA | Start: 32 |
| Tm | 57.976 | 59.731 | End: 134 |
| Size | 27 | 21 | 103 |
| Primer 2 details |
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| Sequence | TCATTCTAAAATTGCCTGTATCTTTGT | AGCACTTGCAGCAGAAATGA | Start: 32 |
| Tm | 57.976 | 58.387 | End: 133 |
| Size | 27 | 20 | 102 |
| Primer 3 details |
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| Sequence | TCATTCTAAAATTGCCTGTATCTTTGT | CAGCACTTGCAGCAGAAATG | Start: 32 |
| Tm | 57.976 | 58.302 | End: 134 |
| Size | 27 | 20 | 103 |
| Primer 4 details |
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| Sequence | TCATTCTAAAATTGCCTGTATCTTTGT | AGCACTTGCAGCAGAAATGAA | Start: 32 |
| Tm | 57.976 | 59.041 | End: 133 |
| Size | 27 | 21 | 102 |
| Primer 5 details |
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| Sequence | TCATTCTAAAATTGCCTGTATCTTTGT | AGCACTTGCAGCAGAAATGAAG | Start: 32 |
| Tm | 57.976 | 60.03 | End: 133 |
| Size | 27 | 22 | 102 |
