ssr_primer
Primer Details for TRINITY_DN80120_c2_g1_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGACGCTATGTGGTTCTT | ATCGGCTGGATATTCTGCGG | Start: 1217 |
| Tm | 60.038 | 60.037 | End: 1343 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TGCGACGCTATGTGGTTCTT | GCTGGATATTCTGCGGTCCA | Start: 1217 |
| Tm | 60.038 | 59.893 | End: 1339 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | CCTGGTTCAAAACGGGCATG | GCTCCGGCTAGCCAACTAAA | Start: 236 |
| Tm | 60.038 | 60.108 | End: 337 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
|---|
| Sequence | ACAGCCGCCATTATCATCGT | TCGCTTTACATTTGCGCGAG | Start: 1605 |
| Tm | 59.893 | 59.905 | End: 1739 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CCGCAGAATATCCAGCCGAT | GAACGTCGCCAGTCCAAATG | Start: 1324 |
| Tm | 60.037 | 59.834 | End: 1532 |
| Size | 20 | 20 | 209 |
(C)12ttcc(A)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGACGCTATGTGGTTCTT | ATCGGCTGGATATTCTGCGG | Start: 1217 |
| Tm | 60.038 | 60.037 | End: 1343 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TGCGACGCTATGTGGTTCTT | GCTGGATATTCTGCGGTCCA | Start: 1217 |
| Tm | 60.038 | 59.893 | End: 1339 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | CCTGGTTCAAAACGGGCATG | GCTCCGGCTAGCCAACTAAA | Start: 236 |
| Tm | 60.038 | 60.108 | End: 337 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
|---|
| Sequence | ACAGCCGCCATTATCATCGT | TCGCTTTACATTTGCGCGAG | Start: 1605 |
| Tm | 59.893 | 59.905 | End: 1739 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CCGCAGAATATCCAGCCGAT | GAACGTCGCCAGTCCAAATG | Start: 1324 |
| Tm | 60.037 | 59.834 | End: 1532 |
| Size | 20 | 20 | 209 |
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