ssr_primer
Primer Details for TRINITY_DN80110_c5_g7_i1 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGACCAGCAGGAATGGGTA | GGAGGGACGGGGAGTTCTAT | Start: 312 |
| Tm | 60.253 | 60.106 | End: 536 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
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| Sequence | TATGCCGCGCTTAGTTCCAT | TACCCATTCCTGCTGGTCCT | Start: 100 |
| Tm | 59.894 | 60.253 | End: 331 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
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| Sequence | ACCTGAACTCCGTCCATTGG | GGAGGGACGGGGAGTTCTAT | Start: 405 |
| Tm | 59.675 | 60.106 | End: 536 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
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| Sequence | AGGACCAGCAGGAATGGGTA | TTTGCTTGTCTGTGGGGAGG | Start: 312 |
| Tm | 60.253 | 60.179 | End: 551 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
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| Sequence | CTCGATCCACTGTGCGCTAA | TACCCATTCCTGCTGGTCCT | Start: 53 |
| Tm | 60.179 | 60.253 | End: 331 |
| Size | 20 | 20 | 279 |
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