ssr_primer
Primer Details for TRINITY_DN80108_c4_g1_i11 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACCCGAAGTCTCAAGCCAA | AACAGAAGTTCGCGATCGGT | Start: 643 |
| Tm | 59.965 | 60.039 | End: 795 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | ACCGATCGCGAACTTCTGTT | CAGAGTTCAACACGCTGCAC | Start: 776 |
| Tm | 60.039 | 60.042 | End: 993 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | GGGCTCATTCGCAATGGAAC | TTGACATCCCTCATTGGCCC | Start: 1095 |
| Tm | 59.899 | 60.033 | End: 1275 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | GGCGGTACTCTATGAGCACC | TCGACTTTCGTCACTGCCAA | Start: 1834 |
| Tm | 59.968 | 59.897 | End: 1966 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | GGCGGTACTCTATGAGCACC | TTCGACTTTCGTCACTGCCA | Start: 1834 |
| Tm | 59.968 | 59.897 | End: 1967 |
| Size | 20 | 20 | 134 |
(T)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACCCGAAGTCTCAAGCCAA | AACAGAAGTTCGCGATCGGT | Start: 643 |
| Tm | 59.965 | 60.039 | End: 795 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | ACCGATCGCGAACTTCTGTT | CAGAGTTCAACACGCTGCAC | Start: 776 |
| Tm | 60.039 | 60.042 | End: 993 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | GGGCTCATTCGCAATGGAAC | TTGACATCCCTCATTGGCCC | Start: 1095 |
| Tm | 59.899 | 60.033 | End: 1275 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | GGCGGTACTCTATGAGCACC | TCGACTTTCGTCACTGCCAA | Start: 1834 |
| Tm | 59.968 | 59.897 | End: 1966 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | GGCGGTACTCTATGAGCACC | TTCGACTTTCGTCACTGCCA | Start: 1834 |
| Tm | 59.968 | 59.897 | End: 1967 |
| Size | 20 | 20 | 134 |
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