ssr_primer
Primer Details for TRINITY_DN80075_c0_g1_i9 |
|---|
| (T)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTGGAAGTGGCTGAGCTG | ATGAGTGTAAACCCAGCGCA | Start: 3614 |
| Tm | 59.964 | 59.964 | End: 3759 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | TGGCAATTCATCGTCGGACA | ATGAGTGTAAACCCAGCGCA | Start: 3638 |
| Tm | 60.037 | 59.964 | End: 3759 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | TGCAACTTGAGCGAGAGAGG | CGCTGTCTAATCGCATGTGC | Start: 2174 |
| Tm | 60.038 | 60.043 | End: 2425 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | TGCAACTTGAGCGAGAGAGG | CGCATGTGCACTTTACGAGG | Start: 2174 |
| Tm | 60.038 | 59.905 | End: 2414 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | CGCCACCTGTATTAGCCCAA | CGGTCAGTATCCTGCCCATC | Start: 1336 |
| Tm | 60.107 | 59.966 | End: 1502 |
| Size | 20 | 20 | 167 |
(TGC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTGGAAGTGGCTGAGCTG | ATGAGTGTAAACCCAGCGCA | Start: 3614 |
| Tm | 59.964 | 59.964 | End: 3759 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | TGGCAATTCATCGTCGGACA | ATGAGTGTAAACCCAGCGCA | Start: 3638 |
| Tm | 60.037 | 59.964 | End: 3759 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | TGCAACTTGAGCGAGAGAGG | CGCTGTCTAATCGCATGTGC | Start: 2174 |
| Tm | 60.038 | 60.043 | End: 2425 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | TGCAACTTGAGCGAGAGAGG | CGCATGTGCACTTTACGAGG | Start: 2174 |
| Tm | 60.038 | 59.905 | End: 2414 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | CGCCACCTGTATTAGCCCAA | CGGTCAGTATCCTGCCCATC | Start: 1336 |
| Tm | 60.107 | 59.966 | End: 1502 |
| Size | 20 | 20 | 167 |
(T)10a(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTGGAAGTGGCTGAGCTG | ATGAGTGTAAACCCAGCGCA | Start: 3614 |
| Tm | 59.964 | 59.964 | End: 3759 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | TGGCAATTCATCGTCGGACA | ATGAGTGTAAACCCAGCGCA | Start: 3638 |
| Tm | 60.037 | 59.964 | End: 3759 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | TGCAACTTGAGCGAGAGAGG | CGCTGTCTAATCGCATGTGC | Start: 2174 |
| Tm | 60.038 | 60.043 | End: 2425 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | TGCAACTTGAGCGAGAGAGG | CGCATGTGCACTTTACGAGG | Start: 2174 |
| Tm | 60.038 | 59.905 | End: 2414 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | CGCCACCTGTATTAGCCCAA | CGGTCAGTATCCTGCCCATC | Start: 1336 |
| Tm | 60.107 | 59.966 | End: 1502 |
| Size | 20 | 20 | 167 |
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