ssr_primer
Primer Details for TRINITY_DN80012_c2_g1_i10 |
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| (C)10ttacgaatcttgattttcctttaatcaaggtacactatttttaaaacaaaactctcttgactcaatttttagctcgatttcc(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTCTCCCTGTTCACGAGG | GAGATCGAGAGCAGCGTGAA | Start: 1514 |
| Tm | 60.037 | 59.9 | End: 1661 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
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| Sequence | GCTTCCTTCCAGCACCTTCT | ATCACGGTGGCAGGATGAAG | Start: 1149 |
| Tm | 59.963 | 60.108 | End: 1264 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
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| Sequence | CGGTCTCCAAAACCACCAGA | AGAAGGTGCTGGAAGGAAGC | Start: 1025 |
| Tm | 59.892 | 59.963 | End: 1168 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
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| Sequence | TCTCCAAAACCACCAGAGCC | AGAAGGTGCTGGAAGGAAGC | Start: 1028 |
| Tm | 59.89 | 59.963 | End: 1168 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | CACGTTTGTCATGCCTGTCG | AGAAGGTGCTGGAAGGAAGC | Start: 1056 |
| Tm | 60.111 | 59.963 | End: 1168 |
| Size | 20 | 20 | 113 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTCTCCCTGTTCACGAGG | GAGATCGAGAGCAGCGTGAA | Start: 1514 |
| Tm | 60.037 | 59.9 | End: 1661 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | GCTTCCTTCCAGCACCTTCT | ATCACGGTGGCAGGATGAAG | Start: 1149 |
| Tm | 59.963 | 60.108 | End: 1264 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | CGGTCTCCAAAACCACCAGA | AGAAGGTGCTGGAAGGAAGC | Start: 1025 |
| Tm | 59.892 | 59.963 | End: 1168 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | TCTCCAAAACCACCAGAGCC | AGAAGGTGCTGGAAGGAAGC | Start: 1028 |
| Tm | 59.89 | 59.963 | End: 1168 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | CACGTTTGTCATGCCTGTCG | AGAAGGTGCTGGAAGGAAGC | Start: 1056 |
| Tm | 60.111 | 59.963 | End: 1168 |
| Size | 20 | 20 | 113 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTCTCCCTGTTCACGAGG | GAGATCGAGAGCAGCGTGAA | Start: 1514 |
| Tm | 60.037 | 59.9 | End: 1661 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | GCTTCCTTCCAGCACCTTCT | ATCACGGTGGCAGGATGAAG | Start: 1149 |
| Tm | 59.963 | 60.108 | End: 1264 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | CGGTCTCCAAAACCACCAGA | AGAAGGTGCTGGAAGGAAGC | Start: 1025 |
| Tm | 59.892 | 59.963 | End: 1168 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | TCTCCAAAACCACCAGAGCC | AGAAGGTGCTGGAAGGAAGC | Start: 1028 |
| Tm | 59.89 | 59.963 | End: 1168 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | CACGTTTGTCATGCCTGTCG | AGAAGGTGCTGGAAGGAAGC | Start: 1056 |
| Tm | 60.111 | 59.963 | End: 1168 |
| Size | 20 | 20 | 113 |
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